Dr. Ruffner is a pediatric allergist whose goal is to improve to care of patients with food allergy syndromes by understanding how the immune system’s interaction with food antigens and the mucosal surfaces are altered in patients with non-IgE mediated food allergies. The Ruffner laboratory studies the genetic and immunologic mechanism that underlie non-IgE mediated food allergies such as eosinophilic esophagitis (EoE) and food-protein induced enterocolitis syndrome (FPIES). The mechanism of non-IgE mediated food allergies are relatively poorly understood compared to food allergen anaphylaxis, which is mediated by IgE. The mission of our research is to improve treatment for patients with these allergies by improving the understanding of the immunologic and genetic causes of these allergic disorders.
The postdoctoral fellow (postdoc) will have a terminal degree PhD, MD, DVM, etc. and will conduct translational pediatric allergy, mucosal immunology, and epigenetics research under the direction/guidance of Dr. Melanie Ruffner at the Children’s Hospital of Philadelphia (CHOP) and University of Pennsylvania (UPenn).
The successful candidate will be dedicated and motivated, have creative thinking and be a driver of her/his research. Several possible project opportunities exist under this project umbrella for the interested candidate, which will build on existing studies using several model systems from within our lab. These include tissue culture model systems of primary and immortalized epithelial cells, flow cytometry and molecular biology assays, as well as analysis of multi-omics datasets such as ChIP-seq, RNA-seq, microarray and genome wide association studies (GWAS), either generated within our lab, affiliated laboratories or from the public domain.
The preferred applicant will have two to three years of working with computational data and commensurate programming proficiency (Unix/Linux, R or Python or Perl, experience with High Performance Computing).
- Conduct independent scholarly research and develop new methods and protocols
- Lead specific projects under PI supervision, with appropriate project planning, recording and evaluation of data
- Acquire technical, lab management and grant writing skills
- Maintain timely, accurate logs of all experiments and any bioinformatic analyses
- Participate in seminars, lectures, poster sessions and presentation at national meetings
- Submit findings in peer-reviewed journals
- Supervise junior lab members
- Assist with other research projects