Seattle Children’s Therapeutics (SCTx) is an exciting new venture within the Seattle Children’s health system that aims to develop and translate novel cellular therapies into groundbreaking clinical trials for children battling the most aggressive forms of cancer. By genetically reprogramming a patient’s own T cells to recognize and kill tumor cells, SCTx seeks to pioneer life-saving cancer immunotherapies developed internally and/or with external partners that may serve as precise alternatives to radiation and chemotherapy.
The successful candidate will join the R&D team within the SCTx. The R&D team is dedicated to the development of cell-based therapeutics with a goal of making better clinical therapies. The work we do is highly cross-disciplinary, intensely collaborative, and incredibly impactful. We are looking for a versatile and highly motivated team member who will assist in engineering next generation CAR T cells and evaluating their performance.
Work will involve primary T cell isolation and culturing, viral or non-viral based gene delivery, transgene expression analysis, in vitro functional assays, multi-parameter flow cytometry, and intracellular cytokine staining. You will also assist in the development of new analytical assays that aim to provide a better understanding of the engineered CAR T cell safety and efficacy. Analyses may include deep genetic analysis, metabolic characterization, T cell signaling, and more. Importantly, we are looking for a creative individual with a willingness to think outside of the box and an ability to work in a dynamic, fast-paced environment. We look forward to hearing from you!
Seattle Children’s Employer Highlights:
• Leader in Pediatric Care: Because of our people, Seattle Children’s is recognized as a leading teaching, research and specialty care center at the forefront of pediatric care.
• Competitive Benefits: We support a healthy work–life balance. Our benefits include employee care, paid time off, health insurance and retirement savings.
• Transportation Discounts: Company bike program, on-site zip cars, unlimited ORCA transit pass, daily commute bonus provided to staff who us alternate methods of travel to/from work, company vanpools.
• Diversity/Inclusion: We strive to maintain an atmosphere that reflects our values of inclusion by providing effective and respectful care compatible with each patient and family’s beliefs, values, and heritage.
– Bachelor’s Degree in Immunology, Cell Biology, Molecular Biology, Bioengineering, or a related field.
– Prior experience in a laboratory setting.
– One year of research experience.
– Experience handling primary T-cells and/or other demanding cell types.
– Experience with viral and non-viral based DNA delivery, molecular cloning, qPCR or ddPCR, western blotting, NGS sequencing, or Nanostring.